All sports: 2022 season
- Austin FC
- Birmingham Legion FC
- CF Montréal
- Central Valley Fuego FC
- Charleston Battery
- Charlotte FC
- Charlotte Independence
- Chattanooga Red Wolves SC
- Chicago Fire FC
- Chicago Fire FC II
- Colorado Rapids
- Colorado Rapids 2
- Colorado Springs Switchbacks FC
- Columbus Crew
- Columbus Crew 2
- D.C. United
- Detroit City FC
- El Paso Locomotive FC
- FC Cincinnati
- FC Cincinnati 2
- FC Dallas
- FC Tucson
- FC Tulsa
- Forward Madison FC
- Greenville Triumph SC
- Hartford Athletic
- Houston Dynamo 2
- Houston Dynamo FC
- Indy Eleven
- Inter Miami CF
- Inter Miami CF II
- LA Galaxy
- Los Angeles FC
- Loudoun United FC
- Louisville City FC
- Memphis 901 FC
- Miami FC
- Michigan Stars FC
- Minnesota United FC 2
- Monterey Bay FC
- Nashville SC
- New England Revolution
- New England Revolution II
- New Mexico United
- New York City FC
- New York City FC II
- New York Red Bulls
- New York Red Bulls II
- North Carolina FC
- North Texas SC
- Oakland Athletics
- Orange County SC
- Orlando City B
- Orlando City SC
- Philadelphia Union
- Philadelphia Union II
- Phoenix Rising FC
- Pittsburgh Riverhounds SC
- Portland Timbers
- Portland Timbers 2
- Racing Louisville FC
- Real Monarchs
- Real Salt Lake
- Richmond Kickers
- Rio Grande Valley FC Toros
- Sacramento Republic FC
- San Antonio FC
- San Diego Loyal SC
- San Jose Earthquakes
- San Jose Earthquakes II
- Seattle Sounders FC
- Sporting Kansas City II
- St. Louis City SC 2
- Tacoma Defiance
- Tampa Bay Rowdies
- Toronto FC
- Toronto FC II
- Whitecaps FC 2